Proteinresearch's Weblog

Lipase Research

proteinresearch — Wed, 14 Nov 2007 22:17:15 +0000

Pancreatic lipase is an enzyme secreted from the pancreas that uses hydrolysis to break a part fat molecules. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat fat droplets. Because the droplets are small, their surface area is greater, allowing the lipase to break apart the fat more effectively. The resulting monomers are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Pancreatic lipase is secreted into the duodenum through the duct system of the pancreas. Normally lipase concentration in serum is very low. Under extreme disruption of pancreatic function, such as pancreatitis or pancreatic adenocarcinoma, the pancreas may begin to autolyse and release pancreatic enzymes into serum. Thus, through measurement of serum concentration of pancreatic lipase, pancreatitis can be diagnosed.

A blood test for lipase is ordered, often along with an amylase test, to help diagnose and monitor acute pancreatitis (inflammation of the pancreas), chronic pancreatitis, and other disorders that involve the pancreas.
Lipase testing is also occasionally used in the diagnosis and follow-up of cystic fibrosis, celiac disease, and Crohn’s disease.

HUMAN PANCREATIC LIPASE ENZYME is commercially available in research and bulk quantities from Lee Biosolutions, Inc at www.leebio.com

Human alpha-1 antitrypsin research

proteinresearch — Mon, 12 Nov 2007 19:57:32 +0000

Lee Biosolutions announces the availability of Alpha-1 antitrypsin Antigen to clinical researchers worldwide. Alpha-1 antitrypsin is a protein produced in the liver that blocks the destructive effects of certain Enzymes.

An Alpha-1 antitrypsin Antigen concentration test is used as a tool to help diagnose the cause of early onset emphysema, especially when a person does not present obvious risk factors, like smoking or exposure to lung irritants such as dust and fumes. Alpha-1 antitrypsin tests are also implemented in diagnosing the cause of persistent jaundice and liver dysfunction indicators, primarily in young children.

According to Lee Biosolutions President Burton Lee, the company continues to introduce new products on a regular basis in order to stay ahead of the demands of the clinical research community.

“Production of proteins like alpha-1 antitrypsin is the result of a need by researchers for pure and consistent products.” Lee said.

If a patient’s alpha-1 antitrypsin concentration is below normal, Alpha-1 antitrypsin Antigen phenotype testing may be ordered. That testing is sometimes followed by DNA testing to establish which Pi gene alleles are present.

Lee Biosolutions also offers affinity purified polyclonal antibodies of goat anti-human alpha-1 antitrypsin and polyclonal chicken anti-human alpha-1 anti-trypsin .

Lee Biosolutions is a leader in the biomarker industry, providing quality products to clinical labs and researchers around the world, developing tomorrow’s cures. LeeBio has provided human proteins used in a number of high profile biomarker studies. For more information or to place an order, contact Lee Biosolutions at 314-968-1091, or visit the company’s website, LeeBio.com.

Prostate Specific Antigen research, Prostate Specific Antigen source

proteinresearch — Fri, 09 Nov 2007 17:29:29 +0000

Prostate-specific antigen (PSA) is an androgen-regulated serine protease produced by both prostate epithelial cells and prostate cancer (PCa) and is the most commonly used serum marker for cancer. Prostate specific antigen (PSA) is a protein produced by the cells of the prostate gland. Prostate-specific antigen is present in small quantities in the serum of normal men, and is often elevated in the presence of prostate cancer and in other prostate disorders.

PSA is a major protein in semen , where its function is to cleave semenogelins in the seminal coagulum. PSA is secreted into prostatic ducts as an inactive 244–amino acid proenzyme (proPSA) that is activated by cleavage of seven N-terminal amino acids. PSA that enters the circulation intact is rapidly bound by protease inhibitors, primarily alpha1-antichymotrypsin, although a fraction is inactivated in the lumen by proteolysis and circulates as free PSA

A PSA blood test is the most effective test currently available for the early detection of prostate cancer. Higher than normal levels of PSA are associated with both localized and metastatic prostate cancer (CaP).

Human Transferrin Research, Holo Transferrin, Apo Transferrin

proteinresearch — Fri, 09 Nov 2007 17:23:40 +0000

Human Transferrin is a plasma protein for iron ion delivery. Human Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron binding domains. Transferrin(TF) is related to other iron binding proteins including lactoferrin . When human transferrin loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and is consequently transported into the cell in a vesicle. The cell will acidify the vesicle, causing human transferrin(TF) to release its iron ions. Each TRANSFERRIN molecule has the ability to carry two iron ions in the ferric form (Fe3+). Commercially purified Human Apo Transferrin and human Holo Transferrin available from diagnostic raw material manufacturers such as Lee Biosolutions, Inc .

Human Lipase research, lipase studies

proteinresearch — Fri, 09 Nov 2007 17:03:16 +0000

Pancreatic lipase is an enzyme secreted from the pancreas that uses hydrolysis to break a part fat molecules. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat fat droplets. Pancreatic LIPASE is secreted into the duodenum through the duct system of the pancreas. Normally lipase concentration in serum is very low. Under extreme disruption of pancreatic function, such as pancreatitis or pancreatic adenocarcinoma, the pancreas may begin to autolyse and release pancreatic enzymes into serum. Thus, through measurement of serum concentration of pancreatic lipase, pancreatitis can be diagnosed.